Journal: International Journal of Molecular Sciences

Article Title: From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different Candida albicans Extracellular Vesicles

doi: 10.3390/ijms26010244

Figure Lengend Snippet: ( a ) Hierarchical heatmap depicting the relative abundance of each protein across the three different types of EVs, with darker shades of red indicating higher relative abundance (measured by NSAF) (protein names shown represent 1 out of every 22 proteins for clarity). ( b ) Zoomed-in view on the region of the heatmap with a higher abundance in cell surface proteins in SC5314 YEVs. Proteins described as immunogenic in the Candida Genome Database (CGD) are marked with an asterisk.

Article Snippet: The NSAF values, which are calculated considering the number of matched peptide spectra (PSMs) and the molecular weight, were used to conduct a clustering heatmap analysis with Rstudio ( a).

Techniques:

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: A) De novo identified cell populations from the software ICGS are shown for each scRNA-Seq platform. The displayed heatmaps were produced by the MarkerFinder algorithm, downstream of the ICGS population predictions, with yellow indicating high relative gene expression and blue or black, low or no gene expression in the associated genes (rows). Prior established embryonic kidney marker genes corresponding to compartments are shown in panel C. Text to the left of each heatmap indicates the statistical enrichment of genes from the Drop-Seq ICGS analysis for the 16 identified populations (MarkerFinder) using the embedded gene-set enrichment analysis tool GO-Elite in AltAnalyze. B–C) t-SNE plot derived from the ICGS heatmaps in panel A, where each dot represents individual cells colored according to its B) ICGS cluster annotation or C) prior established population specific genes. CD: Collecting duct, UT: Ureteric Tip, LOH: Loop of Henle, RV: Renal vesicle, DCSB: Distal comma shaped body, Pod: podocyte, PT: Proximal Tubule, PA: Pre-tubular aggregate, CM: Cap mesenchyme, Endo: Endothelium, NZS: Nephrogenic Stroma, CS: Cortical Stroma.

Article Snippet: Gene set enrichment analysis of ToppGene defined GUDMAP genes was performed using the GO-Elite option in the hierarchical clustering and heatmap viewer user-interface in AltAnalyze ( Chen et al., 2009 ; Zambon et al., 2012 ).

Techniques: Software, Produced, Gene Expression, Marker, Derivative Assay

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: Heatmap with representative cells from the CM: cap mesenchyme, PA: pretubular aggregate, DCSB: distal comma shaped body, MSSB: mid S-shaped body, PT: proximal tubule, LOH: loop of Henle, and Pod: Podocyte clusters from Drop-seq, Chromium 10X Genomics, and Fluidigm 800-cell. The early progenitor CM cells show stochastic expression of markers of multiple lineages. The MSSB cells are more committed and show elevated expression of proximal tubule associated genes and reduced expression of podocyte marker genes.

Article Snippet: Gene set enrichment analysis of ToppGene defined GUDMAP genes was performed using the GO-Elite option in the hierarchical clustering and heatmap viewer user-interface in AltAnalyze ( Chen et al., 2009 ; Zambon et al., 2012 ).

Techniques: Expressing, Marker

Journal: Nature Communications

Article Title: Natural variation of an E3 ubiquitin ligase encoding gene Chalk9 regulates grain chalkiness in rice

doi: 10.1038/s41467-025-61683-4

Figure Lengend Snippet: a The genome-wide association signals for chalky grain rate (CGR) and degree of chalkiness (DC) in the region at 18–21 Mb on chromosome 9 ( x -axis) across two years. Negative log 10 -transformed P values from the linear mixed model are plotted on the y -axis. The horizontal dashed line indicates the genome-wide significance threshold ( P = 1×10 –6 ). P values were determined using a two-sided Wald test and assessed after Bonferroni correction for multiple comparisons. b Linkage disequilibrium (LD) heatmap of the Chalk9 locus region. Pairwise linkage disequilibrium was determined by calculating r 2 (the square of the correlation coefficient between SNPs). c Relative expression level of the 12 candidate genes in the endosperm of eight high-chalky and eight low-chalky varieties at 20 days after flowering (DAF). The 12 predicted genes in the Chalk9 locus region are labeled by I to XII. Data show means ± SD ( n = 8 varieties). P values were calculated for comparisons between high-chalky and low-chalky groups, with each group comprising 8 varieties. d Relative expression level of the candidate gene III ( Chalk9 ) in the endosperm from the selected varieties at 20 DAF. The P value was calculated for the comparison between high-chalky and low-chalky groups, with each group comprising 8 varieties. Data show means ± SD ( n = 3 biological replicates). e Relative expression level of the 12 candidate genes in the leaves of eight high-chalky and eight low-chalky varieties. Data show means ± SD ( n = 8 varieties). In c – e , statistical analysis between high-chalky and low-chalky groups was performed by two-tailed Student’s t -test. Source data are provided as a Source Data file.

Article Snippet: Correlation analysis, heatmap plotting, and volcano plot analysis were performed using BMKCloud ( www.biocloud.net ).

Techniques: GWAS, Transformation Assay, Genome Wide, Expressing, Labeling, Comparison, Two Tailed Test